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KMID : 0380620130450050557
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Korean Journal of Food Science and Technology
2013 Volume.45 No. 5 p.557 ~ p.564
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Isolation and Identification of a Sterol and Three Glucosides from the Peel of Pear (Pyrus pyrifolia Nakai cv. Chuhwangbae)
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Lee Yu-Geon
Cho Jeong-Yong
Lee Hyun-Joo
Lee Yong-Hyun
Lee Sang-Hyun
Han Tae-Ho
Kim Wal-Soo
Park Keun-Hyung
Moon Jae-Hak
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Abstract
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We isolated and identified antioxidants from acidic and neutral ethyl acetate fractions of the peel of pear (Pyrus pyrifolia N. cv. Chuhwangbae). We isolated 4 compounds from the methanol extract, by using 3 different types of column chromatography (Sephadex LH-20, silica gel, and octadecylsilane) and preparative HPLC. We identified the isolated compounds as (S)-(+)-2-cis-abscisic acid O-β-D-glucopyranosyl ester (compound 1), 1-[4-O-β-D-glucopyranosyl]phenyl ethanone (picroside, compound 2), β-sitosterol (compound 3), and β-sitosteryl 3-O-β-D-glucopyranoside (compound 4) by nuclear magnetic resonance analysis. We are the first to report the identification of compounds 1, 2, and 4 from pear.
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KEYWORD
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abscisic acid glucoside, antioxidant, β-sitosterol, β-sitosteryl glucoside, picroside
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